Washington D.C. [USA], July 20 (ANI): A team of researchers has recently identified and described two new frog species found in Ecuador.

"I have been looking at these frogs for years now, so going over the whole process of observing them in their habitats and then analysing them and comparing them under the microscope, to finally naming them is a long, but very satisfying journey," said Urgiles, lead author of the study published in the journal of ZooKeys.

"One of the things that I found most interesting about these guys is that they don't have metamorphosis like a regular frog, but instead they develop entirely inside eggs that adult females deposit in the ground. They really don't need water bodies for their development. Both of the new frog species inhabit high elevation ecosystems in the mountain range over 8,000 feet, so even though we are right there in the equator, it's very cold and windy most of the year," Urgiles explained.

The team of researchers has been studying frogs in Ecuador for the past few years. In 2017, Urgiles found the first new species and named it Pristimantis quintanai. She found the second species Pristimantis cajanuma in 2018. Both were found in the Paramo and montane forest of the southern Ecuadorean Andes.

The frogs are tiny, measuring .8 inch. Pristimantis quintanai females are brown and black and Pristimantis cajanuma are green and black, both easily blending into the foliage. They have a distinct call that is sharp and continuous, sounding like tik-tik-tik-tik.

Urgiles examined DNA samples collected by the international team back in Savage's lab at UCF, generated genetic sequences, and constructed the phylogenetic analysis. Other team members also worked the morphological diagnosis and comparisons with other frogs and acoustic analysis of the frogs' calls.

"In these analyses, we use all of the genetic similarities and differences we find to build phylogenetic trees, and when we find that a 'branch' on the 'tree' has strong support and contains all of the individuals that share the same morphological characteristics, then we have good evidence to describe it as a new species. We used this method, along with vocalisation and location data, to conclude that the two species we describe are distinct from any other species that have ever been characterised," said Assistant Professor Anna Savage.

The work is critical because of the vast diversity that has yet to be discovered in the tropical Andes of South America. In 2018, 13 new species of frogs were documented in the tropical Andes of Ecuador and so far in 2019 five new frogs have been documented.

There are potentially thousands of new plants and animals in the area that may hold the key to other discoveries. It's important to know what is there, to better understand the threats to habitat loss and disease so conservation methods can be established to protect the resources. (ANI)